Little is known of the trial of Ann Pudeator. She was a well-to-do, seventy-one year old widow living in Salem Town in when she was accused. It is recorded that she was examined with Mary Warren near the beginning of April, and that at the girl accused Ann at the same time she accused John Proctor and Alice Parker. It is believed that Mary was fed the testimony against Ann, as she listed dates and events far more specifically than she ever had before.

Ann Pudeator was hanged on Gallows Hill, September 22, 1692.